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比特派下载|gst

作者：比特派下载

2024-03-07 19:03:48

GST是什么费用？怎么计算？ - 知乎

GST是什么费用？怎么计算？ - 知乎切换模式写文章登录/注册GST是什么费用？怎么计算？林凯漫GST英文全称GOODS and SERVICES TAX，即货物服务税，与我国征收VAT (VALUEADDED TAX)营业加值税类似。GST是几乎每个生产和经销阶段有供货或提供服务时征收，唯一享有免GST的项目是食品。供货商和服务机构必须在税务部门注册。他们购买物品或服务时所付的GST将可在他们供货或提供服务给其客户时所征的GST相抵消。澳洲GST:澳州政府征收的税。为商品售价的10%。生活中的几乎样样商品都要征收GST(各个方面的购买行为，包括公交车票之类)。但是部分日常食品，比如面包、牛奶和水果之类不需要征收GST。加拿大GST:加拿大政府全国征收的消费税，大约为5.3%。除了GST，加拿大还有PST(Provincial Sales Tax)大约为8.5%:省消费税。和中国不同，加拿大GST+PST都在消费时候征收消费者，由商家代收，直接打印到收据上。除了农业产品免于收税以外，全部消费品都不得逃税。目前只有美国、加拿大、澳洲有征收此费用。如何计算GST:如果商品包含了GST (GST included )则把商品价格除以11,即可得出GST值;如果商品未包含GST (GST excluded )则把商品价格除以10,即可得出GST值。发布于 2021-04-28 14:39消费税海运国际物流赞同 61 条评论分享喜欢收藏申请

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GST pull-down 技术原理及实验流程 - 知乎

GST pull-down 技术原理及实验流程 - 知乎首发于实验高手养成记切换模式写文章登录/注册GST pull-down 技术原理及实验流程非知名研究僧科研服务老兵GST-Pull Down作为体外检测蛋白间直接互做的行之有效的方法，可用于证实预测的蛋白质-蛋白质相互作用的存在，也可用作未知的蛋白质-蛋白质相互作用的初始筛选测定法。 GST-Pull Down的原理 GST Pull-Down实验基于GST（glutathione-S-transferase），即谷胱甘肽-S-转移酶蛋白，可以与谷胱甘肽（Glutathione，GSH）结合。将GSH固定于琼脂糖珠上，形成GSH-琼脂糖珠，将已知蛋白X与GST融合表达，获得的GST-X可与GSH-琼脂糖珠结合，若环境中存在与X蛋白互做的蛋白Y，则会形成“琼脂糖珠-GSH-GST-X-Y”复合物，与X蛋白互做的蛋白即可被分离并检测。GST-Pull Down示意图GST-Pull Down原理GST Pull-Down实验方法（验证预测的两个蛋白的互做）实验设计Pull Down实验体系编号1234A-GST+--+B-His-+-+GST--+-实验设计1.IPTG诱导pET28a-B-His及pGEX-4T-1-A载体融合蛋白表达1.1 表达载体转化至大肠杆菌Rosseta;1.2 挑取转化平板上的单克隆接种于含50 μg/mL Amp的3 mL LB培养液的试管中，37℃摇菌过夜，制备种子液;1.3 次日按1:100接种于50 μg/mL Amp的30 mL LB培养液中，37℃摇至菌体OD600为0.8;1.4 向菌液中加入IPTG至终浓度为0.5 mM，11~28℃摇床培养，诱导融合蛋白表达4~8h;1.5 将菌液离心弃上清，用 PBS重悬菌体沉淀，超声波破碎，离心取上清;1.6 吸取少量上清用于SDS-PAGE检测目的蛋白表达情况。2.细胞破碎及蛋白纯化采用超声破碎，GST柱亲和纯化及Ni柱亲和纯化，分别获得纯化后的目的蛋白。2.1 GST柱纯化（1）利用低压层析系统，细胞裂解上清液上样至GST Binding-Buffer预平衡的GST亲和层析柱;（2）用GST Binding-Buffer冲洗层析柱，至流出液OD280值到达基线;（3）用GST Elution-Buffer洗脱目的蛋白，收集流出液;（4）收集的蛋白溶液加入透析袋中，使用Tris-HCl透析过夜;（5）进行SDS-PAGE分析和WB分析。2.2 Ni柱纯化（1）利用低压层析系统，细胞裂解上清液上样至Ni-IDA Binding-Buffer预平衡的Ni-IDA -Sepharose Cl-6B亲和层析柱;（2）用Ni-IDA Binding-Buffer冲洗层析柱，至流出液OD280值到达基线;（3）用Ni-IDA Washing-Buffer冲洗层析柱，至流出液OD280值到达基线;（4）用Ni-IDA ELution-Buffer洗脱目的蛋白，收集流出液;（5）收集的蛋白溶液加入透析袋中，使用Tris-HCl进行透析过夜;（6）进行SDS-PAGE分析和WB分析。3.GST-Pull Down实验使用GST Protein Interaction Pull-Down Kit (购自Thermo公司)3.1平衡树脂（GST）(1)混匀Immobilized Glutathione，彻底重悬树脂;(2)各取100 µL 50% 树脂悬浮液到4个离心管中，1250 ×g离心2 min弃上清;(3)用500 µl PBS重悬树脂，1250 ×g离心2 min移除上清，重复3次。3.2蛋白互作(1)按照按照表1分别将A-GST蛋白和B-His蛋白加入到谷胱甘肽树脂中，将混合物在4°C旋转混匀孵育过夜;(2)1250 ×g离心5 min后，弃上清（上清要取净）;(3)用500 µL PBS重悬树脂。1250×g 离心2 min弃上清。重复3次。3.3诱饵蛋白和靶蛋白复合物的洗脱(1)每管加入50 µL Elution Buffer;(2)4°C 旋转混匀孵育20 min;(3)1250 ×g 离心2 min弃上清，离心下来的液体置于冰上;(4)取20 µL样品进行SDS-PAGE和WB检测。发布于 2021-04-15 09:32蛋白质蛋白纯化蛋白质工程赞同 1017 条评论分享喜欢收藏申请转载文章被以下专栏收录实验高手养成记分享实验方法、技能、实验心得，加速科研

谷胱甘肽S-转移酶（GST）的生理功能和应用 - 每日生物评论

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新加坡消费税（GST）解析 - 知乎

新加坡消费税（GST）解析 - 知乎切换模式写文章登录/注册新加坡消费税（GST）解析丹妮毕业于英国赫特福德大学金融系，在新加坡生活二十年的武汉美美。新加坡消费税（Goods and Services Tax，GST）是在包括进口商品在内的购买或消费商品及服务时所缴纳的税务。消费税（GST）由新加坡海关征收。在其他国家，消费税被称为增值税或VAT。新加坡目前消费税的税率是7%。（根据新加坡的经济，政府支出和其他税收收入，计划在2021年至2025年之间的某个时间将2％的利率从7％提高到9％。）1. GST注册要求根据新加坡税务局的相关规定，如果公司销售应纳税货物及服务的营业额超过100万新币或者估计将会超过100万新币，就必须在履行该责任的30天内强制注册消费税。如果公司在未来的12个月内，商品或服务营业额少于1百万新币，或已不再提供应纳税商品和服务，则可以申请取消注册消费税公司。不满足此条件的公司也可以选择自愿注册消费税。自愿注册的批准由新加坡税务局酌情决定，一旦获得批准GST的注册，必须至少维持2年。在注册后的首两年内，不能取消消费税注册公司身份，除非已经停供商品或服务，或已停止经营。消费税申报标准程序为每季度进行一次，也可以要求每月或每半年申报一次，但仍需要税务局进行批准。如果大部分的货物或服务主要是出口，或向全球供应的零税率供应品（zero-rated supplies），则不需要注册消费税。注册的商家必须向消费者收取消费税，非消费税注册商家，则不得要求消费者在购买商品时支付消费税。2. GST计费和收费一旦注册了消费税之后，就需以现行7%的税率为企业的供应征收消费税。消费税计费和收费被称为销项税（Output Tax），收取后必须呈给新加坡税务局。企业在商业上的采购和开支（包括进口商品）所产生的消费税，被称为进项税(Input Tax)。如果该企业符合索取进项税的条件，可以就采购和支出索取进项税。即，公司可以用向消费者所收取的消费税来补回购买货物是所缴纳的消费税。当进项税多过销项税时，公司可以向税务局申请消费税退款补回差额，若当销项税超过进项税时，则需要将差额交给税务局。这种进项税抵免机制，确保只有在每个供应链阶段的增值额征税。3. GST供应类型新加坡一般有四种供应类型标准税率供应（消费税7% *）- 大部分本地商品和服务销售属于这一类别。零税率供应（消费税0%）- 出口商品和服务被归为国际服务豁免供应（消费税不适用）– 出售和租赁无家具的住宅物业，进口和本地供应贵重金属的投资，金融服务等消费税范围之外的供应（消费税不适用）– 商品在海外进行销售，私人交易等4.允许索回进项税的条件是GST注册公司，有关货物或服务必须已经提供给您或货物已由您进口；商品或服务将被使用，或将用于您的业务；对于本地采购，在申请索回进项税时，需要有效的税务发票对于进口，必须有进口许可并表明该公司是有关货物的进口商；进项税额直接属于应税供应（即标准供应和零税率供应），或者是消费税范围外的供应（若在新加坡生产就是应税供应）。在第26及27条消费税条例下，并没有被禁止索回的进项税。5. 不允许索回的进项税消费税（普通）条例第26及27条不允许以下支出申索进项税：提供给您的员工的家属或亲属的福利；使用私家轿车的成本和费用；体育和娱乐俱乐部收取的会费（包括转会费）；公司车所产生的费用，在1998年4月1日更新或延长拥车证；1999年7月1日或之后租用汽车所产生的费用；工作人员的医疗保险费，除非根据“工伤赔偿法令”，或根据“劳资关系法”的任何集体协议，该保险或赔偿是强制性的。涉及博彩、抽奖、彩票、水果机器或机会游戏的任何交易。6. 进口服务的消费税的新规定从2020年1月1日起,新加坡实施如下对进口服务征税的新制度: 反向收费机制征税进口服务的企业对企业供应（B2B）的反向纳税制度，如市场推广服务、资讯科技服务、会计服务等。对消费者(B2C)进口数字服务的海外供应商登记制度（OVR），如音乐和视频流服务、应用程序、在线订阅费用等。以反向纳税方式对B2B进口服务征税当海外供应商提供B2B服务给新加坡当地消费税注册企业时, 消费税登记收款人必须按其进口服务的价值计算消费税。对于非消费税注册企业来说，从2020年1月1日起，如果企业在12个月内进口服务的总价值超过新加坡币100万元，并且即使企业已经登记了消费税，企业也没有资格获得全额进项税收抵免，那么企业可能需要根据新的消费税注册规则进行消费税注册。一旦企业注册了消费税，将被要求在应税供应和进口服务中计入消费税，而进口服务需收取反向费用。被影响的企业：主要是金融机构、混合和住宅房地产开发商、投资控股公司获得股息和从公司之间贷款取得的利息收入，以及组织提供免费或补贴活动，如慈善机构、自愿福利机构(vwo)、非营利组织(非营利组织)、医院和教育机构。适用于反向收费机制征税的企业：消费税注册企业从海外供应商采购服务，或者，非消费税注册企业。例如，在过去12个月内从海外供应商取得超过100万新元的服务，即使登记了消费税，也无权获得全部进项税额抵免。通过海外供应商注册制度对B2C数字服务征税新加坡境外地区的企业如果达到以下标准，则需要在新加坡注册商品及服务税：年度全球营业额超过100万新元;和为新加坡客户提供超过100,000新元的B2C数码服务。一旦注册GST后，企业就需要对在新加坡向客户提供的数字服务的B2C供应收取和结算消费税费用。电子市场运营商在某些情况下，无论您是电子市场的本地或海外运营商，您都可以被视为海外供应商通过您的市场提供的数码服务的供应商。在这种情况下，企业需要包含这些服务的价值，以确定商品及服务税登记责任。如果企业需对GST注册负责或已经过GST注册，则除了提供数码服务外，还需要向代表海外供应商的新加坡客户收取有关B2C数码服务供应的GST费用。数码支付代币自2020年1月1日起，使用或提供数码支付代币作为任何商品（法定货币或其他数码支付代币除外）的支付，均被视为消费税供应。出于消费税目的，以下数码支付代币供应都被视为免税供应：将数码支付令牌交换为法定货币或其他数字支付代币提供借贷数码支付代币数码支付代币的资格包括以下所有特征：它是一个单位；其设计是可替代的；它不是以任何货币计价的，也不是由其发行人与任何货币挂钩的；可以电子方式转让、存储或交易；它是或打算成为公众或部分公众所接受的交换媒介，对其使用没有任何实质性限制；但不包括：金钱；如果被提供，是金融服务的免税供应；赋予权利以从一个或多个特定人士接收或指导商品或服务的供应的任何东西，并且在使用该权利后不再充当交换媒介的功能。数字支付令牌的示例包括比特币、以太币、莱特币、达世币、门罗币、瑞波币和Zcash。发布于 2021-09-01 18:43税收税务增值税赞同 4添加评论分享喜欢收藏申请

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Goods and Services Tax (GST): Definition, Types, and How It's Calculated

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Goods and Services Tax (GST)

Understanding the GST

Dual Goods & Services Tax Structures

Critiques of the GST

Example

GST vs. GST Tax

GST FAQs

The Bottom Line

Fiscal Policy

Tax Laws

Goods and Services Tax (GST): Definition, Types, and How It's Calculated

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What Is the Goods and Services Tax (GST)?

The goods and services tax (GST) is a value-added tax (VAT) levied on most goods and services sold for domestic consumption. The GST is paid by consumers, but it is remitted to the government by the businesses selling the goods and services.

Critics point out, however, that the GST may disproportionately burden people whose self-reported income are in the lowest and middle income brackets, making it a regressive tax. These critics argue that GST can therefore exacerbate income inequality and contribute to social and economic disparities. In order to address these concerns, some countries have introduced GST exemptions or reduced GST rates on essential goods and services, such as food and healthcare. Others have implemented GST credits or rebates to help offset the impact of GST on lower-income households.

Goods and services tax should not be confused with the generation-skipping trust, also abbreviated GST (and its related taxation, GSTT).

Key Takeaways

The goods and services tax (GST) is a tax on goods and services sold domestically for consumption.The tax is included in the final price and paid by consumers at point of sale and passed to the government by the seller.The GST is usually taxed as a single rate across a nation.Governments prefer GST as it simplifies the taxation system and reduces tax avoidance.Critics of GST say it burdens lower income earners more than higher income earners.

Understanding the Goods and Services Tax (GST)

The goods and services tax (GST) is an indirect federal sales tax that is applied to the cost of certain goods and services. The business adds the GST to the price of the product, and a customer who buys the product pays the sales price inclusive of the GST. The GST portion is collected by the business or seller and forwarded to the government. It is also referred to as Value-Added Tax (VAT) in some countries.

Most countries with a GST have a single unified GST system, which means that a single tax rate is applied throughout the country. A country with a unified GST platform merges central taxes (e.g., sales tax, excise duty tax, and service tax) with state-level taxes (e.g., entertainment tax, entry tax, transfer tax, sin tax, and luxury tax) and collects them as one single tax. These countries tax virtually everything at a single rate.

France was the first country to implement the GST in 1954; since then, an estimated 140 countries have adopted this tax system in some form or another. Some of the countries with a GST include Canada, Vietnam, Australia, Singapore, United Kingdom, Spain, Italy, Nigeria, Brazil, and India.

Dual Goods and Services Tax Structures

Only a handful of countries, such as Canada and Brazil, have a dual GST structure. Compared to a unified GST economy where tax is collected by the federal government and then distributed to the states, in a dual system, the federal GST is applied in addition to the state sales tax. In Canada, for example, the federal government levies a 5% tax and some provinces/states also levy a provincial state tax (PST), which varies from 8% to 10%. In this case, a consumer's receipt will clearly have the GST and PST rate that was applied to their purchase value.

More recently, the GST and PST have been combined in some provinces into a single tax known as the Harmonized Sales Tax (HST). Prince Edward Island was the first to adopt the HST in 2013, combining its federal and provincial sales taxes into a single tax. Since then, several other provinces have followed suit, including New Brunswick, Newfoundland and Labrador, Nova Scotia, and Ontario.

Critiques of the GST

A GST is generally considered to be a regressive tax, meaning that it takes a relatively larger percentage of income from lower-income households compared to higher-income households. This is because GST is levied uniformly on the consumption of goods and services, rather than on income or wealth.

Lower-income households tend to spend a larger proportion of their income on consumables, such as food and household goods, which are subject to GST. As a result, GST can disproportionately burden lower-income households.

Because of this. some countries with GST are discussing possible adjustments that might make the tax more progressive, which takes a larger percentage from higher-income earners.

Example: India's Adoption of the GST

India established a dual GST structure in 2017, which was the biggest reform in the country's tax structure in decades. The main objective of incorporating the GST was to eliminate tax on tax, or double taxation, which cascades from the manufacturing level to the consumption level.

For example, a manufacturer that makes notebooks obtains the raw materials for, say, Rs. 10, which includes a 10% tax. This means that they pay Rs. 1 in tax for Rs. 9 worth of materials. In the process of manufacturing the notebook, the manufacturer adds value to the original materials of Rs. 5, for a total value of Rs. 10 + Rs. 5 = Rs. 15. The 10% tax due on the finished good will be Rs. 1.50. Under a GST system, the previous tax paid can be applied against this additional tax to bring the effective tax rate to Rs. 1.50 – Rs. 1.00 = Rs. 0.50.

In turn, the wholesaler purchases the notebook for Rs. 15 and sells it to the retailer at a Rs. 2.50 markup value for Rs. 17.50. The 10% tax on the gross value of the good will be Rs. 1.75, which the wholesaler can apply against the tax on the original cost price from the manufacturer (i.e., Rs. 15). The wholesaler's effective tax rate will, thus, be Rs. 1.75 – Rs. 1.50 = Rs. 0.25.

Similarly, if the retailer's margin is Rs. 1.50, his effective tax rate will be (10% x Rs. 19) – Rs. 1.75 = Rs. 0.15. Total tax that cascades from manufacturer to retailer will be Rs. 1 + Rs. 0.50 + Rs. 0.25 + Rs. 0.15 = Rs. 1.90.

India has, since launching the GST on July 1, 2017, implemented the following tax rates:

A 0% tax rate applied to certain foods, books, newspapers, homespun cotton cloth, and hotel services.A rate of 0.25% applied to cut and semi-polished stones.A 5% tax on household necessities such as sugar, spices, tea, and coffee.A 12% tax on computers and processed food.An 18% tax on hair oil, toothpaste, soap, and industrial intermediaries.The final bracket, taxing goods at 28%, applies to luxury products, including refrigerators, ceramic tiles, cigarettes, cars, and motorcycles.

The previous system, with no GST, implies that tax is paid on the value of goods and margin at every stage of the production process. This would translate to a higher amount of total taxes paid, which is carried down to the end consumer in the form of higher costs for goods and services. The implementation of the GST system in India is, therefore, a measure that is used to reduce inflation in the long run, as prices for goods will be lower.

Goods and Services Tax vs. Generation-Skipping Transfer Tax

The goods and services tax (GST) should not be confused with the generation-skipping transfer tax (GSTT Tax), and they are not at all related to one another.

The former is a sort of VAT tax added to the purchase of goods or serves. Meanwhile, the generation skipping transfer tax (GST Tax) is a flat 40% federal tax on the transfer of inheritances from one's estate to a beneficiary who is at least 37½ years younger than the donor. The GST Tax prevents wealthy individuals from avoiding estate taxes through naming younger beneficiaries (e.g., grandchildren).

Who Has to Pay GST?

In general, goods and services tax (GST) is paid by the consumers or buyers of goods or services. Some products, such as from the agricultural or healthcare sectors, may be exempt from GST depending on the jurisdiction.

How Is GST Calculated?

The goods and services tax (GST) is computed by simply multiplying the price of a good or service by the GST tax rate. For instance, if the GST is 5%, a $1.00 candy bar would cost $1.05.

What Are the Benefits of the GST?

The GST can be beneficial as it simplifies taxation, reducing several different taxes into one straightforward system. It also is thought to cut down on tax avoidance among businesses and reduces corruption.

Are VAT and GST the Same?

Value-added tax (VAT) and goods and services tax (GST) are similar taxes that are levied on the sale of goods and services. Both VAT and GST are also indirect taxes, which means that they are collected by businesses and then passed on to the government as part of the price of the goods or services.However, there are some key differences between the two. VAT is primarily used in European countries and is collected at each stage of the production and distribution process, while GST is used in countries around the world and is collected only at the final point of sale to the consumer. VAT is generally applied to a wider range of goods and services than GST, and the rate of VAT and GST can vary depending on the type of goods or services being sold and the country in which they are sold.

The Bottom Line

The goods and services tax (GST) is a type of tax levied on most goods and services sold for domestic consumption in many countries. It is paid by consumers and remitted to the government by the businesses selling the goods and services. Some countries have introduced GST exemptions or reduced GST rates on essential goods and services or have implemented GST credits or rebates to help offset the impact of GST on lower-income households. The GST is often a single rate tax applied throughout a country and is preferred by governments because it simplifies the taxation system and reduces tax avoidance. In dual GST systems, such as those in Canada and Brazil, the federal GST is applied in addition to a state sales tax. The GST has been identified by critics as regressive and can potentially place a relatively higher burden on lower-income households.

Article Sources

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editorial policy.

Parliament of Australia. "The Proposed GST: Impact on the Distribution of Income."

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022: France," Download "Download This Tax Guide," Page 595.

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022," Download "Download This Tax Guide," Page Preface.

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022: Brazil," Download "Download This Tax Guide," Pages 238-241.

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022: Canada," Download "Download This Tax Guide," Pages 296-298.

Government of Canada. "General Information for GST/HST Registrants."

Prince Edward Island, Canada. "Backgrounder: Harmonized Sales Tax (HST)."

Motherboard. "GST may be a regressive tax, but part of a progressive system: Shawn Huang & WP's Louis Chua clash over GST."

Financial Review. "The GST can be progressive: here's how."

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022: India," Download "Download This Tax Guide," Page 748.

Government of India, Department of Revenue. "Goods and Services Tax One Country One Tax One Market FAQs on Goods and Services Tax (GST)."

Ernst & Young. "Worldwide VAT, GST and Sales Tax Guide 2022: India," Download "Download This Tax Guide," Pages 752-753.

Related Terms

Cascade Tax: Definition, Examples, and Alternatives

A cascade tax is a system that imposes sales taxes on products at each successive stage in their progress, from the beginning raw material to the end stage of consumer purchase.

Consumption Tax: Definition, Types, vs. Income Tax

A consumption tax is a tax on the purchase of a good or service. It can also be a system taxing based on consumption rather than on added value to the economy (income tax).

Harmonized Sales Tax (HST): Definition as Canadian Sales Tax

The harmonized sales tax is a hybrid of the Canadian goods and services tax and provincial sales tax applied to goods and services.

Tax Revenue: What It Is, How It Works, Types

Reliance on an income tax versus a consumption tax is one big difference between U.S. revenue sources and those of other countries. Learn the options.

Indirect Tax: Definition, Meaning, and Common Examples

An indirect tax is passed off to the consumer as part of the purchase price of a good or service. Learn how indirect taxes work and when you may face them.

Tax Rate Definition, Effective Tax Rates, and Tax Brackets

A tax rate is a percentage at which an individual or corporation is taxed. A progressive tax rate imposes additional, higher rates as income increases.

Cascade Tax: Definition, Examples, and Alternatives

What Are Some Examples of a Value-Added Tax (VAT)?

Consumption Tax: Definition, Types, vs. Income Tax

Harmonized Sales Tax (HST): Definition as Canadian Sales Tax

Tax Revenue: What It Is, How It Works, Types

Indirect Tax: Definition, Meaning, and Common Examples

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GST-tagged Fusion Protein Purification | Thermo Fisher Scientific - PH

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Home›Life Sciences›Protein Biology›Protein Sample Preparation›Protein Purification›Recombinant Protein Purification›GST-tagged Fusion Protein PurificationGST-tagged Fusion Protein PurificationSee Navigation‹Recombinant Protein PurificationHis-tagged Fusion Protein Purification

›GST-tagged Fusion Protein Purification

›Epitope-tagged Protein Purification

›Reduced glutathione (GSH), when immobilized as a ligand to agarose or other chromatography supports, enables high-yield and high-quality purification of recombinant proteins expressed as fusions with glutathione S-transferase (GST). GST-fusion proteins are purified with high yield because of the 12-atom GSH linker, which minimizes steric hindrance. We offer glutathione magnetic beads and agarose resins in multiple formats to accommodate a variety of needs, from high- throughput screening, batch, pilot, and process purification. Superflow agarose resins have undergone extensive chemical characterization.More formats—magnetic beads, magnetic agarose, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable fusion protein purification from microgram to kilogram scales.High performance—resins are designed to maximize protein yield and reduce background.Economical—pricing is similar to or better than other suppliers.

Choose a product for GST-tagged fusion protein purification

Product typePierce Glutathione Magnetic Agarose BeadsPierce Glutathione Agarose ResinPierce Glutathione Superflow ResinBead or resin size10–40 μm45–165 μm60–160 μmStatic binding capacity≥12 mg/mL~40 mg/mL~30 mg/mLDynamic binding capacity*N/A~10.5 mg/mL~10 mg/mLMaximum linear flow rate*N/A800 cm/hr1,200 cm/hrSupportMagnetite-embedded 6% beaded agarose6% beaded agaroseHighly crosslinked 6% beaded agaroseNo. of reuses0525Formats availableloose beadsloose beads, pre-packed spin columns and kits, chromatography cartridgesloose beadsRecommended application scalehigh-throughput batchbatch, pilotbatch, pilot, processOrder product(s)7860116100 (loose resin)16109 (1 mL cartridge)16110 (5 mL cartridge)16111 (spin plate)25236

Additional Pierce Glutathione Agarose products

ResinFormatResin per columnColumn sizeCollection tube sizeCat. No. GlutathioneSpin Column0.2 mL0.8 mLMicrocentrifuge16103GlutathioneSpin Column1 mL2 mL15 mL conical16104GlutathioneSpin Column3 mL10 mL50 mL conical16105GlutathioneSpin Column Kit0.2 mL0.8 mLMicrocentrifuge16106GlutathioneSpin Column Kit1 mL2 mL15 mL conical16107GlutathioneSpin Column Kit3 mL10 mL50 mL conical16108

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海湾GST，一站式消防整体解决方案提供商

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